Their fold-change values were within a range of −1.25-fold < x < 1.25-fold. As a result, we identified seven constitutively expressed genes among 8453 genes across all conditions. diazoefficiens under multiple environmental conditions. Here, we analyzed previous gene expression data for B. By revealing such HKGs, the normalization of gene expression would be more robust, reliable, and consistent. The goal of this study is to identify and confirm HKGs in Bradyrhizobium diazoefficiens, a nitrogen fixing bacterium which forms a symbiotic relationship with soybean. Since the normalization of gene expression data depends on baseline expression of HKGs, it is important to identify and verify true HKGs for the qRT-PCR analysis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) has been used to measure gene expression. These HKGs are usually involved in basic cellular functions and their expression should remain at relatively constant levels. Reference genes, also referred to as housekeeping genes (HKGs), play an important role in gene expression analysis by serving as an internal control.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |